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In the context of the unstoppable trend of artificial intelligence, science and technology have become the theme of the times. Will the rapid development of modern technology, such as biotechnology and artificial intelligence, dehumanize us? Can a machine have human consciousness? In his novel Klara and the Sun, Kazuo Ishiguro criticizes the arrogance of technological rationality and the arrogance of anthropocentrism from the perspective of a “non-human” robot. The relationship between humans and machines has become a problem that humans need to re-examine. With the help of post-humanism, this paper aims to explore the physical changes and behavioral actions of robots and humans in the novel to reveal the “split” between man and machine and the “self-deception” of humans in the novel, so as to finally trigger thinking about how humans and machines can coexist harmoniously at the juncture between humans and posthumans, and provide reference for the future society between humans and non-humans.